WebFeb 16, 2008 · The images were processed by Adobe Photoshop Software. The number of sMyCH-, fMyHC-, or both sMyHC- and fMyHC-positive myotubes containing more than two nuclei was counted. The data represent the mean±SEM. Significance was tested by Student’s t-test. All comparisons of statistical significance were set at P-values of P < 0.05. WebThe Igf-2 pattern closely correlates with FMyHC from E13.5 and peaks at E15.5 when over 90% of FMyHC+ myotubes also contain Igf-2. Igf-2 lags FMyHC and it is absent from muscle myotubes until E13 ...
Muscular dystrophy begins early in embryonic development …
WebNov 3, 2024 · The primary antibodies for γH2AX (Cell Signaling), fast-type Myosin Heavy Chain (FMyHC) (Abcam), and Lamin A/C (Santa Cruz) were used at a 1:100 to 1:300 dilution. The cells were analyzed via fluorescence microscopy (Nikon Instruments Inc. Melville, NY) and photographed at 4–40× magnification. WebJul 14, 2024 · The elevated expression of MyoG and MEF2c in rainbow trout white muscle indicates a positive influence of dietary Se on the differentiation of myoblasts, which could also be evidenced by the upregulated expression of fMyHC, a marker for the sarcomeric assembly in the late stages of myoblast differentiation . The promoted proliferation of … grandy curler
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WebJul 1, 2024 · Relative expression of fMyHC to sMyHC mRNA in red and white muscle of rainbow trout. Expression was normalized to the warm acclimated trout. For white … WebJul 7, 2011 · Monoclonal antibody against fMyHC was purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA). Polyclonal antibodies against myogenin and BMP-2 were … WebMar 19, 2001 · Muscle fibers attach to laminin in the basal lamina using two distinct mechanisms: the dystrophin glycoprotein complex and the α7β1 integrin. Defects in these linkage systems result in Duchenne muscular dystrophy (DMD), α2 laminin congenital muscular dystrophy, sarcoglycan-related muscular dystrophy, and α7 integrin congenital … grandy creek grocery concrete