Fusion index myoblast
WebJun 10, 2024 · The fusion index is commonly used in muscle cell culture assays to determine the amount of myoblast fusion [6,7,8,9]. To this end, nuclei are visualized using DNA binding compounds like Hoechst, and myotubes are stained using fluorescent labelled antibodies for structural muscle protein, mainly myosin heavy chain (MyHC) among others. WebApr 1, 2024 · (B) The fusion index of differentiated immortal myoblast cultures was determined by the percentage of the number of nuclei in myotubes (>2 myonuclei) to the total number of nuclei in desmin-positive cells (n=3 for each condition, >1000 nuclei counted per experiment). (C) Nuclear RNA aggregates of expanded-CUG or -CCUG repeats in …
Fusion index myoblast
Did you know?
WebSep 15, 2024 · Interestingly, myoblast fusion was significantly decreased in response to co-culture with macrophages, while the effect of fibroblasts on fusion was density … WebAug 31, 2024 · Compared to WT, SOD1-mutant myoblasts had a significantly lower fusion index (p < 0.001) (Fig. 2F). Additionally, the number of myotubes per area in each culture was quantified as another measure ...
WebMay 1, 2010 · Cell-cell fusion is a crucial and highly regulated event in the genesis of both form and function of many tissues. One particular type of cell fusion, myoblast fusion, is a key cellular process that shapes the formation and repair of muscle. Despite its importance for human health, the mechanisms un … WebMay 11, 2024 · The myoblast fusion index was calculated as the ratio of the number of nuclei inside myotubes compared to the total number of nuclei × 100 at day 10 of myogenic differentiation via ImageJ software. Co-localization was quantified with the Coloc2 plugin for the ImageJ software program ...
WebJun 22, 2024 · The fusion index was determined to estimate the fusion capacity of the cultured myoblasts within the different stimulation conditions. The fusion index was found to be slightly increased in the 22 h per day stimulation condition (77 ± 7%) compared to statically cultured myotubes (69 ± 9%) (Figures 2A,B). WebDec 16, 2024 · The fusion index is indicative of the level of differentiation from myoblasts into myotubes ... Snyman C, Niesler C. Analysis and quantification of in vitro myoblast …
WebMyoblast fusion is essential for formations of myofibers, the basic cellular and functional units of skeletal muscles. Recent genetic studies in mice identified two long-sought …
WebMar 16, 2024 · In order to experimentally evaluate myoblast fusion in vitro, a fusion index is calculated based on detection of immunofluorescence by microscopy. Fluorescently labeled antibodies are used to detect muscle fiber structural proteins such as myosin … ŽTÔ•!D ÑŸÀ ÊЛ3f §¶;™‚HŠ]oú²=Åcu˜Š`úD„ ŠêN%A ¶=¶ê … BioTechniques is a MEDLINE-indexed, peer-reviewed, open access journal that … Digital Features in our Journals. All our journals can feature a range of digital … BioTechniques is a MEDLINE-indexed, peer-reviewed, open access journal that … frontline tracking freightWebDec 1, 2016 · Myoblast fusion, which is essential for muscle development, regeneration, and repair, can be assessed in vitro via the calculation of a fusion index. Traditionally, … frontline tpsWebAug 9, 2024 · To validate this assay, myoblast fusion index measurements and immunohistochemistry (see below) were performed in parallel. Fig. 1.Decreased myoblast fusion and myotube formation in cerebral palsy (CP). A: example of a normalized differentiation assay performed over ~42 h with the iCELLigence RTCA system. Green … frontline tracking infoWebNov 4, 2024 · The differentiation index and fusion index were counted. Zfp422-KO myoblasts coundn't fuse to form myotube. e Zfp422 mRNA level in C2C12 cells after … frontline toy soldiersWebDownload scientific diagram Comparison of myotube density and fusion index after C2C12 treatment. C2C12 cells were differentiated for 4 days in the presence of 10 μM prostaglandin D2 (PGD2), 10 ... frontline toxicity in dogsWebThe differentiation of C2C12 cells was studied over a period of seven days and was quantified; we report the differentiation parameters of (1) fusion index, (2) degree of maturation, (3) alignment, and (4) response to electrical pulse stimulation (EPS). Hybrid structures with the smallest arc degree (hybrid 30°) showed the best results for all ... ghost pacer costWebFeb 17, 2024 · The QKI genes encode RNA-binding proteins regulating cell proliferation, differentiation, and apoptosis. The Goat QKI has six isoforms, but their roles in myogenesis are unclear. In this study, the six isoforms of the QKI gene were overexpressed in goat myoblast. Immunofluorescence, qPCR and Western blot were used to evaluate the … frontline trading hallam