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Mcherry-muc2

WebUsing RedMUC2-Tg mice, which express a human MUC2 fused to mCherry, and originally developed by Hansson and colleagues, Citation 51 Nystrom et al. showed an intercrypt … Web23 feb. 2024 · We constructed four expression vectors of mCherry or mCherry fusions containing a variety of resistance markers and promoters, which were then integrated, together with two previous mCherry expression vectors, in T. mentagrophytes via Agrobacterium tumefaciens -mediated transformation ( At MT).

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Web22 jun. 2024 · Fluorescence Detection of mCherry in Recombinant WCFS1. Recombinant WCFS1 carrying different expression vectors were, respectively, grown in MRS broth for … Web25 jan. 2024 · In the mouse, Muc2 is the predominant intestinal secreted mucin in health, and deficiency of Muc2 leads to spontaneous inflammation and susceptibility to infection. … incl. or inc https://heritagegeorgia.com

The Nlrp6 inflammasome is not required for baseline colonic inner …

Web通过使用mCherry-MUC2转基因小鼠来分选和分离GC，这些作者生成了转录组和蛋白质组图谱来表征小肠和结肠中的GC表达景观。单细胞转录组分析揭示了每个组织区域中几个不 … WebAnti-MUC2 antibody [C3], C-term (GTX100664) GeneTex Back Primary Antibodies Secondary Antibodies Antibody Panels ELISA Antibody Pairs & Kits Isotype Controls … WebProduct Note. Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or … incorporating a plc

Addgene: pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

Category:A sentinel goblet cell guards the colonic crypt by triggering Nlrp6 ...

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Mcherry-muc2

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Web9 aug. 2024 · To determine which of these methionine residues function as an ATIS that still renders a functional mCherry protein, we designed three versions of mCherry (V1, V2, … WebOccludin是一种膜整合蛋白，在紧密连接（tight junction, TJ）细胞旁通透性屏障的形成和调节中发挥作用。. 细胞旁转运（Paracellular transport）是指分子在相邻上皮细胞之间运输，而紧密连接可调节细胞旁路渗透性并维持细胞极性。. Occludin当细胞缺乏紧密连接时，可 …

Mcherry-muc2

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Weban mCherry–human MUC2–expressing transgenic mouse (RedMUC298trTg)revealedsecretedMuc2 onlyintreatedtissues(Fig.1D).Asmucusexpands … WebAnti-MUC2 antibody [SPM296] (GTX34865) GeneTex Back Primary Antibodies Secondary Antibodies Antibody Panels ELISA Antibody Pairs & Kits Isotype Controls Proteins & Peptides Slides Lysates Serums & Plasmas Reagents Research Kits Research Tools Back Cancer Tumor Markers Neuroscience Cell Biology Metabolism Epigenetics …

WebmCherry 679 .. 1389 = 711 bp 236 amino acids = 26.7 kDa. Product: monomeric derivative of DsRed fluorescent protein. mammalian codon-optimized: ori 4020 .. 4608 = 589 bp. high-copy-number ColE1/pMB1/pBR322/pUC origin of replication: ori 4020 .. 4608 = 589 bp. high-copy-number ... WebMS2 Mutation MS2: V75E/A81G Promoter human UbC Tags / Fusion Proteins V5 (N terminal on insert) mCherry (C terminal on insert) Cloning Information Cloning method …

Web2024年4月19日讯/ 生物谷 BIOON/---本周又有一期新的Science期刊（2024年4月16日）发布，它有哪些精彩研究呢？. 让小编一一道来。. 图片来自Science期刊 ... WebSummaryIntracellular pH (pHi) dynamics is increasingly recognized to regulate myriad cell behaviors, including proliferation, migration, differentiation, and transformation. Here we report a new finding that pHi dynamics also regulates adult stem

WebThis was achieved by acquiring the total distal colonic epithelium from transgenic mice expressing mCherry-tagged MUC2 (RedMUC2 98trTg) and sorting the goblet cell …

WebTLR-ligands induce Muc2 secretion in specific GCs Intestinal GCs are more functionally heterogeneous than previously expected (reflected processes happening ... incorporating a partnershipWebmCherry is een lid van de mFruits-familie van monomere rode fluorescerende eiwitten (mRFP's). Als een RFP was mCherry afgeleid van DsRed van Discosoma … incl. of all taxesWebK. Castillo, ... C. Hetz, in Methods in Enzymology, 2024 2.1 Design of AAV2_mCherry-GFP-LC3 Vectors. The whole mCherry-GFP-LC3 expression cassette was excised from a plasmid kindly provided by Terje Johansen's laboratory, and inserted into a shuttle proviral plasmid, containing both the AAV2/2 inverted terminal repeats and the 1.6-kb … incorporating a prec in ontarioOriginal RFP: DsRed First Generation RFP: mRFP1 Second Generation RFPs: mStrawberry, mOrange, dTomato mFruit are second-generation monomeric red fluorescent proteins (mRFPs) that have improved brightness and photostability compared to the first-generation mRFP1. Their emission and excit… incorporating a pdf into a word documentWeb17 apr. 2024 · 通过使用mCherry-MUC2转基因小鼠来分选和分离GC，这些作者生成了转录组和蛋白质组图谱来表征小肠和结肠中的GC表达景观。 单细胞转录组分析揭示了每个 … incorporating a personWebCells were considered selected after greater than 95% of non-transduced control mK4 cells plated to similar density as the transduced mK4 cells were dead. mK4 cells transduced with only vectors expressing EGFP, mCherry, and tdTomato (without puromycin resistance) were flow cytometry sorted with a 100 µm nozzle at 25 PSI using a BD Biosciences … incorporating a rugby clubWebNational Center for Biotechnology Information incl. synonym